Embryogenesis induction of carrot (Daucus carota L.) in isolated microspore culture

Haploid technologies are used to create homozygous lines for accelerated breeding. We aimed optimize the technology using isolated microspore culture in vitro obtain doubled haploids of carrot (Daucus carota L.).
 studied two varieties with different responsiveness embryogenesis, Altajskaya lakomka and Breeding line 17. Carrot microspores were from buds cultivated liquid nutrient media supplemented an antibiotic activated carbon vitro. They exposed thermal treatments.
 The experiment showed benefits combining cold pre-treatment (5°C 1 day) heat shock (32°C 2 days). induction embryogenesis on NLN-13 medium was twice as high MSm-13 medium. use 1% 0.5% agarose increased yield embryoids by more than 1.5 times. 100 mg/L ampicillin found be most efficient concentration. After 30 days cultivation under optimized conditions, 161.3 44.0 per Petri dish cultivar 17, respectively.
 is determined type duration stress, composition medium, a sorbent, addition β-lactam antibiotics, explant treatment. Our enabled us haploid carrots during year, these included breeding process F1 hybrids.